Measurement of myeloperoxidase (MPO; EC 1.11.1.7) activity is often used as a marker of neutrophil infiltration into tissues. However, most enzymatic assays for MPO are susceptible to interference from other peroxidases (including eosinophil peroxidase, EPX) and hemoproteins (such as hemoglobin and
Luminol Chemiluminescent Assay of Cytochrome b5
โ Scribed by T. Kamidate; N. Kida; T. Kamataki; H. Watanabe
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 275 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0003-2697
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## Abstract Cytochrome __b__~5~, a 17โkDa hemeprotein associated primarily with the endoplasmic reticulum of eukaryotic cells, has long been known to augment some cytochrome P450 monooxygenase reactions, but the mechanism of stimulation has remained controversial. Studies in recent years have clari
## Abstract The fate of newly synthesized cytochrome b~5~ was studied in rat hepatocytes. Using an antibody specific for microsomal cytochrome b~5~, we found newly synthesized microsomal cytochrome b~5~ in both mitochondria and a mitochondria associated membrane fraction as well as in microsomes. N