Luminol assay for microdetermination of superoxide dismutase activity: Its application to human fetal blood

✍ Scribed by Trung Pham Huu; Claude Marquetty; Catherine Pasquier; Jacques Hakim

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 512 KB
• Volume: 142
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90491-3

✦ Synopsis

A microtechnique for determining the superoxide dismutase activity in erythrocytes is described. This technique involves the inhibition of luminol-enhanced chemiluminescence of superoxide anion generated by xanthine-xanthine oxidase. Measurements required a steadystate chemiluminescence whether superoxide dismutase was present or absent; the level of luminescence was correlated to enzyme activity. Superoxide dismutase activity measured by this technique was 836 + 112 &g of hemoglobin for whole blood and 834 + 109 rg/g of hemoglobin for erythrocytes. When the reference technique was applied to larger amounts of blood, the results were 862 + 58 and 858 +-116 &g of hemoglobin for whole blood and washed erythrocytes, respectively. The enzymatic activity of superoxide dismutase from fetal blood (obtained by venipuncture in utero and of 19-26 weeks gestational age) was similar to that of adult blood, when measured by the new technique. o 1984 Academic PXSS, IX.