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Lowry method of protein quantification: Evidence for photosensitivity

✍ Scribed by Janet M. Dawson; Paul L. Heatlie

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
209 KB
Volume
140
Category
Article
ISSN
0003-2697

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✦ Synopsis

Despite reports of its susceptibility to various interfering factors, the Folin Phenol protein quantification method of O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951, J. Biol. Chem. 193, 265-275) remains the most convenient and accurate method for routine protein determinations. Our findings indicate that the Lowry assay is also photosensitive which can result in a discrepancy of up to 10% in estimated protein concentrations, unless appropriate precautions are taken.

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Polyacrylamide gel interference of the l
Polyacrylamide gel interference of the lowry method for protein determination
✍ P.A. Fields; L.H. Larkin πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 406 KB

Certain reagents utilized in the formation of polyacrylamide gels are shown to interfere in the Lowry assay for protein. Acrylamide (3-30%) and potassium ferrocyanide (O.OOlS-0.0105%) produced a linear response in color formation. Both compounds are capable of reducing the phenol reagent in the abse