Low-temperature synthesis of 2′-deoxyadenosine using immobilized psychrotrophic microorganisms

✍ Scribed by J. Fernández-Lucas; L.A. Condezo; M.A. Quezada; J.V. Sinisterra

• Book ID: 101727860
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 203 KB
• Volume: 100
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.21756

✦ Synopsis

Abstract

Selective biocatalyzed synthesis of 2′‐deoxyadenosine from 2′‐deoxypyrimidine nucleosides was carried out using free or immobilized whole cells. The reaction was performed at 57°C without secondary reactions. Two psychrotrophic microorganisms, Bacillus psychrosaccharolyticus and Psychrobacter immobilis, are described for the first time as active and specific strains for the synthesis of 2′‐deoxyadenosine. Adenosine deaminase activity was not detected. Whole cells were immobilized in different matrixes. Calcium alginate and calcium pectate gave the best biocatalysts. The synthesis of 2′‐deoxyadenosine follows an apparent first order kinetic expression. External mass transfer control was negligible as deduced from k~s~, N~A~, and Ω values. Internal mass transfer was the rate controlling step according to η~T~ and ϕ values. Biotechnol. Bioeng. 2008;100: 213–222. © 2007 Wiley Periodicals, Inc.