Low temperature box and tissue handling device for embedding biological tissue for immunostaining in electron microscopy

A simple, low-cost device for processing

A simple, low-cost device for processing and embedding tissues at sub-zero temperatures

✍ Wayne Shoemaker; Craig Hayes Jr.; Thomas E. Phillips 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB

## Abstract A simple apparatus to maintain tissues samples at sub‐zero temperatures during dehydration, infiltration, and polymerization is described. The device uses a conventional siphon‐type carbon dioxide gas cylinder to maintain an aluminum block at temperatures as low as −35°C for over 15 hou