𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Low-power composite CPMG HSQMBC experiment for accurate measurement of long-range heteronuclear coupling constants

✍ Scribed by Sándor Boros; Katalin E. Kövér

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
182 KB
Volume
49
Category
Article
ISSN
0749-1581

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A modified version of CPMG‐HSQMBC pulse scheme is presented for the measurement of long‐range heteronuclear coupling constants. The method implements adiabatic inversion and refocusing pulses on the heteronucleus. Low‐power composite 180° XY‐16 CPMG pulse train is applied on both proton and X nuclei during the evolution of long‐range couplings to eliminate phase distortions due to co‐evolution of homonuclear proton–proton couplings. The pulse sequence yields pure absorption antiphase multiplets allowing precise and direct measurement of the ^n^J~XH~ coupling constants regardless from the size of the proton‐proton couplings. The applicability of the method is demonstrated using strychnine as a model compound. The selective 1D version of the method is also presented. Copyright © 2011 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Simplified Multiplet Pattern HSQC-TOCSY
Simplified Multiplet Pattern HSQC-TOCSY Experiment for Accurate Determination of Long-Range Heteronuclear Coupling Constants
✍ Wiktor Koźmiński 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 76 KB

A new two-dimensional pulse sequence for accurately determining heteronuclear coupling constants is presented. It is derived from HSQC and HECADE techniques with B 0 gradient coherence selection. The main feature of the proposed method is spectra with only one component of the IS doublet; i.e., the

One- and two-dimensional gradient-select
One- and two-dimensional gradient-selected HSQMBC NMR experiments for the efficient analysis of long-range heteronuclear coupling constants
✍ R. Thomas Williamson; Brian L. Márquez; William H. Gerwick; Katalin E. Kövér 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 240 KB 👁 2 views

Three new gradient-selected NMR experiments for the analysis of long-range heteronuclear coupling constants are presented. They are demonstrated on a series of compounds including sucrose, strychnine and a complex macrolide, phormidolide, isolated from a marine cyanobacterium. These 1D and 2D hetero

Sensitivity Enhancement of a Two-Dimensi
Sensitivity Enhancement of a Two-Dimensional Experiment for the Measurement of Heteronuclear Long-Range Coupling Constants, by a New Scheme of Coherence Selection by Gradients
✍ Daniel O. Cicero; Gaetano Barbato; Renzo Bazzo 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 74 KB

In this work we present a new pulse sequence for the measurement of long-range heteronuclear coupling constants in which the optimization of coherence selection by pulsed field gradients offers a net increase in sensitivity. This type of experiments is extremely valuable for conformational studies o