𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Low-light imaging technology in the life sciences

✍ Scribed by Hooper, C. E. ;Ansorge, R. E. ;Rushbrooke, J. G.

Publisher
John Wiley and Sons
Year
1994
Weight
832 KB
Volume
9
Category
Article
ISSN
0884-3996

No coin nor oath required. For personal study only.

✦ Synopsis

Photon imaging is an increasingly important technique for the measurement and analysis of chemiluminescence and bioluminescence. New high-performance low-light level imaging systems have recently become available for the life sciences. These systems use advances in camera design and digital image processing and are n o w being used for a wide range o f luminescence applications. They offer good sensitivity for photon detection and large dynamic range, and are suitable for quantitative analysis. This is achieved using a range of software techniques including image arithmetic, histogramming or summing regions of interest, feature extraction and multiple image processing for kinetics or assay screening. Improvements in imageprocessing hardware and software have increased the usefulness o f these systems in the biosciences.

Low-light imaging is a rapid and non-invasive method for the sensitive detection and analysis of luminescent assays. As such it offers a powerful and sensitive tool for investigating processes, both at the cellular level (/uc and lux reporter genes, intracellular signalling) and for measurement of macro samples (immunoassays, gels and blots, tissue sections).

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