Low-level CO2 laser–induced release of 51chromium from canine 2C5 gliosarcoma cells

✍ Scribed by David W. Van Kooten; Dr. Robert J. Maciunas; Robert S. Carver

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 396 KB
• Volume: 13
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.1900130504

✦ Synopsis

Abstract

Recently, interest has grown in the area of low‐power laser effects upon tissues. We used a ^51^Cr cell labeling technique with glioma tissue to better understand these effects. Canine 2C5 gliosarcoma cells with intracellular ^51^Cr were exposed to CO~2~ laser in the range of 0.2 to 3.0 J/cm^2^. Correlative analysis of the data indicated that there is a strong direct relationship between laser fluence and the percent of total intracellular ^51^Cr released from the glioma cells with a coefficient of correlation (r) of +0.93. The calculated standard error of the correlation coefficient was ± 0.06 and the coefficient of determination (r^2^) was 0.86. These results indicate that the ^51^Cr cell labeling technique is a useful method for quantifying the low‐power laser effects on the integrity of the cell membrane of gliosarcoma cells in vitro. However, further investigation is needed to clarify the specific mechanisms by which the CO~2~ laser induces changes upon these cells. © 1993 Wiley‐Liss, Inc. © 1993 Wiley‐Liss, Inc.