Effects of nimodipine and dexamethasone on low-level co2 laser-induced release of 51chromium from canine 2c5 gliosarcoma cells

✍ Scribed by David W. van Kooten; Robert J. Maciunas; Robert S. Carver

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 396 KB
• Volume: 15
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.1900150409

✦ Synopsis

Abstract

Low‐energy penumbral irradiation of surgical lasers may produce undesirable effects on surrounding tissues. This study used a ^51^Cr cell labeling technique to determine if gliosarcoma cells could be therapeutically protected prior to their exposure to low‐power laser irradiation. Canine 2C5 gliosarcoma cells with intra‐cellular ^51^Cr were treated with nimodipine and/or dexamethasone and then exposed to low‐power levels of CO~2~ laser. The ^51^Cr was released from the cells in a dose‐dependent fashion following exposure to laser energy. Correlative analysis of the data indicated that a strong direct relationship between laser fluence and ^51^Cr release did exist for controls and drug‐treated groups with coefficients of correlation r ≥ +0.90 and coefficients of determination r^2^ ≥ 0.82. However, comparison of the data from the drug‐treated and control groups found that there was no significant difference between them (P >.05). Therefore, no protective or detrimental effects were observed with the use of nimodipine and/or dexamethasone on the gliosarcoma cells as tested in this system. Further investigation is necessary in order to define the mechanisms by which low‐power level lasers affect these cells. These effects do not appear to be mediated through localization of mechanisms to the cell membranes or their constituent Ca^2+^channels. © 1994 Wiley‐Liss, Inc.