𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Long-term T-cell-mediated immunity to epstein-barr virus in man. I. Complete regression of virus-induced transformation in cultures of seropositive donor leukocytes

✍ Scribed by D. J. Moss; A. B. Rickinson; J. H. Pope

Publisher
John Wiley and Sons
Year
1978
Tongue
French
Weight
782 KB
Volume
22
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Peripheral blood mononuclear cells from donors of known serological status with respect to EB virus were exposed to the virus in vitro and then cultured at various cell concentrations. All cultures from nine seronegative adult and 12 foetal donors gave rise to cell lines following subculture 4 weeks post infection. In contrast, seropositive donor cultures seeded at the higher cell concentrations developed foci of proliferating EBNA‐positive cells within the first 1–2 weeks but thereafter regressed completely and subcultures made after 4 weeks never gave rise to cell lines. Out of 18 seropositive donors tested, 15 showed regression in all cultures seeded at 10^6^ cells/ml and above, and with the other three donors a proportion of replicate cultures regressed. T‐cell depletion and reconstitution experiments showed that the effect was absolutely dependent upon the presence in the cultures of T cells from these seropositive donors. The results strongly suggest that the regression phenomenon is an in vitro expression of long‐term T‐cell‐mediated immunity to EB virus which the large majority, if not all, infected individuals possess.

📜 SIMILAR VOLUMES

Long-term T-cell-mediated immunity to Ep
Long-term T-cell-mediated immunity to Epstein-Barr virus in man. II. Components necessary for regression in virus-infected leukocyte cultures
✍ A. B. Rickinson; D. J. Moss; J. H. Pope 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 744 KB

## Abstract Regression of EB‐virus‐induced transformation occurs exclusively in cultures of leukocytes from seropositive donors. Studies have shown that the strength of regression could be assayed in terms of the proportion of T cells which must be added to the autologous EB virus‐infected T‐cell‐d

Long-term T-cell-mediated immunity to Ep
Long-term T-cell-mediated immunity to Epstein-Barr virus in man. III. Activation of cytotoxic T cells in virus-infected leukocyte cultures
✍ D. J. Moss; A. B. Rickinson; J. H. Pope 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 819 KB

## Abstract Experiments have been conducted to determine the role played by immune T cells in the regression of EB‐virus‐induced transformation which is exclusively seen in leukocyte cultures from seropositive donors. Kinetic studies suggest that, In virus‐infected cultures from such donors, a popu

Long-term T-cell-mediated immunity to ep
Long-term T-cell-mediated immunity to epstein-barr virus in man. IV. Development of T-cell memory in convalescent infectious mononucleosis patients
✍ A. B. Rlckinson; D. J. Moss; J. H. Pope; N. Ahlberg 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 French ⚖ 650 KB

## Abstract Under appropriate culture conditions, EB virus infection of lymphocytes from seropositive donors leads to regression of transformation, and this was shown previously to be due to activation in a secondary immune response of T lymphocytes inhibitory for the autologous lymphoblastoid cell