𝔖 Bobbio Scriptorium
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Long-term preservation of active luminous bacteria by lyophilization

✍ Scribed by Janda, Ivan ;Opekarová, Miroslava

Publisher
John Wiley and Sons
Year
1989
Weight
179 KB
Volume
3
Category
Article
ISSN
0884-3996

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✦ Synopsis

A simple method for long-term preservation of luminous bacteria is described. Cells of Vibrio fischeri, Photobacterium leiognathi and four strains of P. phosphoreum were suspended in a protective medium of low ionic strength (1% NaCI) supplemented with 15% lactose and 2% soluble starch, and lyophilized. The freeze-dried preparations were sealed under vacuum and stored at 4°C. Luminous bacteria were resuscitated after six months by adding 2% NaCl up t o the original volume. The rehydrated cells exhibited 16-28% of initial bioluminescence so that they could be used for a microbial test of toxicity (the Microtox test). This method is also useful for maintaining luminous bacteria in strain collections.

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