𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Long-chain acyl-coenzyme a synthetase in rat brain myelin

✍ Scribed by K. K. Vaswani; Dr. R. W. Ledeen

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
575 KB
Volume
17
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

Long-chain acyl-CoA synthetase (EC 6.2.1.3), an enzyme(s) that activates fatty acids prior to incorporation into phospholipids and other substances, has been detected in highly purified myelin from rat brain stem. The high levels relative to microsomes (11% and 15% for oleate and arachidonate, respectively) tended to preclude contamination by the latter membrane as the source of activity. Additional evidence came from sequential purification and mixing experiments. Km values were not appreciably different for the two substrates with the two membranes, but Vmax values were approximately 2-4-fold greater for arachidonate in both membranes. Triton X-100 increased activity somewhat in myelin but not in microsomes; with arachidonate as substrate it reduced activity in the latter. Heat inactivation studies and pH profiles suggested the presence of two different enzymes, as previously shown for other tissues.

πŸ“œ SIMILAR VOLUMES

Continuous Recording of Long-Chain Acyl-
Continuous Recording of Long-Chain Acyl-Coenzyme A Synthetase Activity Using Fluorescently Labeled Bovine Serum Albumin
✍ Erland J.F. Demant; Birthe T. NystrΓΈm πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 100 KB

The fluorescence-based long-chain fatty acid probe BSA-HCA (bovine serum albumin labeled with 7-hydroxycoumarin-4-acetic acid) is shown to respond to binding of long-chain acyl-CoA thioesters by quenching of the 450 nm fluorescence emission. As determined by spectrofluorometric titration, binding af

Long-chain fatty Acyl-CoA synthetase enz
Long-chain fatty Acyl-CoA synthetase enzymatic activity in rat liver cell nuclei
✍ Ana Ves-Losada; Rodolfo R. Brenner πŸ“‚ Article πŸ“… 1996 πŸ› Springer 🌐 English βš– 443 KB

A long-chain fatty acyl-CoA synthetase that catalyzes the activation of long-chain fatty acids as thioesters of CoA, was described in rat liver nuclei. This is the first step for further metabolization of fatty acids in the cell. Up to now, it has been shown that long-chain fatty acyl-CoA synthetase

Isolation and Quantitation of Long-Chain
Isolation and Quantitation of Long-Chain Acyl-Coenzyme A Esters in Brain Tissue by Solid-Phase Extraction
✍ J. Deutsch; E. Grange; S.I. Rapoport; A.D. Purdon πŸ“‚ Article πŸ“… 1994 πŸ› Elsevier Science 🌐 English βš– 259 KB

Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and deter