𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Locus control region mediated regulation of adult β-globin gene expression

✍ Scribed by Shermi Liang; Babak Moghimi; Thomas P. Yang; John Strouboulis; Jörg Bungert

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
335 KB
Volume
105
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Many genes residing in gene clusters and expressed in a differentiation or developmental‐stage specific manner are regulated by locus control regions (LCRs). These complex genetic regulatory elements are often composed of several DNAse I hypersensitive sites (HS sites) that function together to regulate the expression of several cis‐linked genes. Particularly well characterized is the LCR associated with the β‐globin gene locus. The β‐globin LCR consists of five HS sites that are located upstream of the β‐like globin genes. Recent data demonstrate that the LCR is required for the association of the β‐globin gene locus with transcription foci or factories. The observation that RNA polymerase II associates with the LCR in erythroid progenitor or hematopoietic stem cells which do not express the globin genes suggests that the LCR is always in an accessible chromatin configuration during differentiation of erythroid cells. We propose that erythroid specific factors together with ubiquitous proteins mediate a change in chromatin configuration that juxtaposes the globin genes and the LCR. The proximity then facilitates the transfer of activities from the LCR to the globin genes. In this article we will discuss recent observations regarding β‐globin locus activation with a particular emphasis on LCR mediated activation of adult β‐globin gene expression. J. Cell. Biochem. 105: 9–16, 2008. © 2008 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Both locus control region and proximal r
Both locus control region and proximal regulatory elements direct the developmental regulation of β-globin gene cluster
✍ Xin-jun Ji; De-pei Liu; Dong-dong Xu; Lei Li; Jing Wang; Chih-chuan Liang 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 233 KB

Using ligation-mediated PCR and in vivo footprinting methods to study the status of DNA-protein interaction at hypersensitive site 2 of locus control region and ␤ maj promoter of erythroid cells of fetal liver and adult bone marrow, we found that during different developmental periods, the status of

Human growth hormone 1 (GH1) gene expres
Human growth hormone 1 (GH1) gene expression: Complex haplotype-dependent influence of polymorphic variation in the proximal promoter and locus control region
✍ Martin Horan; David S. Millar; Jürgen Hedderich; Geraint Lewis; Vicky Newsway; N 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 325 KB 👁 1 views

The proximal promoter region of the human pituitary expressed growth hormone (GH1) gene is highly polymorphic, containing at least 15 single nucleotide polymorphisms (SNPs). This variation is manifest in 40 different haplotypes, the high diversity being explicable in terms of gene conversion, recurr

Study of the single nucleotide polymorph
Study of the single nucleotide polymorphism (SNP) at the palindromic sequence of hypersensitive site (HS)4 of the human β-globin locus control region (LCR) in Indian population
✍ Ritushree Kukreti; Chandrika B-Rao; Swapan Kr Das; Madhusnata De; Geeta Talukder 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 54 KB 👁 2 views

## Abstract LCR, a genetic regulatory element, was examined in β‐thalassemia patients who do not show any mutation in the β‐globin genes. We sequenced LCR‐HS2, HS3, and HS4 in samples from 16 such patients from the Indian population and found only one SNP A‐G in the inverted repeat in HS4. A signif

Variegated expression and delayed retina
Variegated expression and delayed retinal pigmentation during development in transgenic mice with a deletion in the locus control region of the tyrosinase gene
✍ Estela Giménez; Patricia Giraldo; Glen Jeffery; Lluís Montoliu 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 407 KB

## Abstract Summary: Deletion of the tyrosinase locus control region (LCR) in transgenic mice results in variegated expression in the skin. Here we investigate the pigmentation pattern of other tissues that express tyrosinase: iris, choroid, and retina in the same animals. A mosaic distribution of

Oxidative stress enhances AP-1 and NF-κB
Oxidative stress enhances AP-1 and NF-κB-mediated regulation of β2-Glycoprotein I gene expression in hepatoma cells
✍ Wen-Chin Chiu; Chun-Jung Chen; Tzong-Shyuan Lee; Zit-Jie Chen; Pei-Hsin Ke; An-N 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 452 KB

b 2 -Glycoprotein I (b 2 -GPI), also known as apolipoprotein H, is a plasma glycoprotein with poorly defined gene regulation. The aim of this study was to clarify the role of oxidative stress in b 2 -GPI gene regulation and determine the essential transcription element regulating b 2 -GPI expression