Both locus control region and proximal regulatory elements direct the developmental regulation of β-globin gene cluster
✍ Scribed by Xin-jun Ji; De-pei Liu; Dong-dong Xu; Lei Li; Jing Wang; Chih-chuan Liang
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 233 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Using ligation-mediated PCR and in vivo footprinting methods to study the status of DNA-protein interaction at hypersensitive site 2 of locus control region and  maj promoter of erythroid cells of fetal liver and adult bone marrow, we found that during different developmental periods, the status of DNA-protein interaction at both hypersensitive site 2 and  maj promoter changed significantly, and indicated that locus control region might function through a looping mechanism to regulate the expression of downstream genes, and that distal regulatory elements (locus control region, hypersensitive sites) as well as proximal regulatory elements (promoter, enhancer) of -globin gene cluster participate in the regulation of developmental specificity.