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Location of disulfide bonds in antithrombin III

✍ Scribed by Zhongrui Zhou; David L. Smith

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
425 KB
Volume
19
Category
Article
ISSN
1076-5174

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✦ Synopsis

Proteolytic digests of human antithrombin 111 (ATIII) have heen analyzed by a combination of reversed-phase high-performance liquid chromatography and fast atom bombardment (FAB) mass spectrometry for disulfidecontaining peptides which are diagnostic for disulfide linkages in ATIII. These results indicate that disulfide bonds join Cys 8 and Cys 128, Cys 21 and Cys 95, and Cys 247 and Cys 430. In addition, these results demonstrate that systematic searches of the amino acid sequence of a large protein for segments with molecular weights matching FAB mass spectral information is a viable method for identifying peptides independent of the specificity of the enzyme used to fragment the protein.

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