A new method for labeling antibodies which involves selenol-catalyzed reduction of native disulfide bonds in antibodies to generate thiol groups, which then are labeled using thiol-reactive reagents, is described. The reduction and labeling steps of this rapid procedure are carried out in one vessel
Reagents for Rapid Reduction of Native Disulfide Bonds in Proteins
β Scribed by R. Singh; G.M. Whitesides
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 367 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0045-2068
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