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LOCATION OF BINDING SITES ON THE (Na.K)-ATPase FOR FLUORESCEIN-5′-ISOTHIOCYANATE AND OUABAIN

✍ Scribed by Lewis Cantley; Cynthia T. Carilli; Robert A. Farley; David M. Perlman


Book ID
118719794
Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
128 KB
Volume
402
Category
Article
ISSN
0890-6564

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Preparation of Na,K-ATPase Specifically
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Specific labeling is required for energy transfer measurements and to avoid artifacts in the use of fluorophores as reporter groups. Therefore, a method for specific modification by one of the most popular reagents for P-type ATPases (fluorescein 5-isothiocyanate) has been developed. Sulfhydryl reag