𝔖 Bobbio Scriptorium
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Localizing steroid dehydrogenase activity on acrylamide gels: The perils of “histochemistry”

✍ Scribed by James L. O'Conner; Dean P. Edwards; Edwin D. Bransome Jr.

Book ID
102982611
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
743 KB
Volume
78
Category
Article
ISSN
0003-2697

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✦ Synopsis

Two methods were compared for the detection of solubilized human placental 3P-hydroxysteroid dehydrogenase activity in polyacrylamide gels following electrophoresis:

(i) nitroblue tetrazolium-nicotinamide adenine dinucleotide (NBT-NAD) coupled histochemical incubations of entire gels and (2) direct radiochemical assay of 1.5-mm gel slices. Both procedures utilized pregnenolone as enzyme substrate. The histochemical method showed "nothing dehydrogenase" activity in that gels developed identical banding patterns in the presence and absence of substrate. The method also consistently failed to indicate enzyme activity at loci easily determined by radiochemical assay.

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