Reaction for the Localization of Strictosidine Glucosidase Activity on Polyacrylamide gels

✍ Scribed by T. J. C. Luijendijk; L. H. Stevens; R. Verpoorte

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 437 KB
• Volume: 7
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(199601)7:1<16::aid-pca280>3.0.co;2-h

✦ Synopsis

A simple and specific reaction for the activity of the enzyme strictosidine glucosidase in polyacrylamide gels is described. The sensitivity of the method is high, with activity values as low as 60 fKataVmm' being detected.