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Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization

✍ Scribed by Mary E. Harper; Grady F. Saunders

Publisher
Springer
Year
1981
Tongue
English
Weight
905 KB
Volume
83
Category
Article
ISSN
0009-5915

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✦ Synopsis

Recombinant lambda bacteriophage clone H3 containing a human DNA segment of 14.9 kb present in one or two copies per haploid genome was isolated. In situ hybridization to human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling (53% of cells) of band p36 of chromosome 1, such that 22% of all chromosomal grains were located on this region. Hybridization was dependent upon the presence of dextran sulfate in the hybridization mixture and was not affected by repetitive DNA competitor. These results demonstrate localization of a single copy sequence on human metaphase chromosomes.

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