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Localization and nucleotide specificity of Blastocystis succinyl-CoA synthetase

โœ Scribed by Karleigh Hamblin; Daron M. Standley; Matthew B. Rogers; Alexandra Stechmann; Andrew J. Roger; Robin Maytum; Mark Van Der Giezen

Book ID
110834906
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
687 KB
Volume
68
Category
Article
ISSN
0950-382X

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Succinyl-CoA synthetase from Saccharomyces cerevisiae was partially purified (20-fold) with a yield of 44%. The Michaelis-Menten constants were determined: Km (succinate) = 17 mM; Km (ATP) = 0.13 mM; Km (CoA) = 0.03 mM. The succinyl-CoA synthetase has a molecular weight of about 80000 dalton (as det