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Purification and properties of succinyl-CoA synthetase from Jerusalem artichoke mitochondria

โœ Scribed by J.M. Palmer; R.T. Wedding


Book ID
114301069
Publisher
Elsevier Science
Year
1966
Weight
503 KB
Volume
113
Category
Article
ISSN
0926-6593

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Partial purification and characterizatio
โœ H. Schwartz; H. -O. Steitz; F. Radler ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 511 KB

Succinyl-CoA synthetase from Saccharomyces cerevisiae was partially purified (20-fold) with a yield of 44%. The Michaelis-Menten constants were determined: Km (succinate) = 17 mM; Km (ATP) = 0.13 mM; Km (CoA) = 0.03 mM. The succinyl-CoA synthetase has a molecular weight of about 80000 dalton (as det