Lithographically structured biological recognition structures for immunosensing and single cell analytics

✍ Scribed by Köhler, Oliver ;Kowalewski, Philipp ;Ziegler, Christiane

• Book ID: 105366037
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 365 KB
• Volume: 208
• Category: Article
• ISSN: 0031-8965
• DOI: 10.1002/pssa.201000945

✦ Synopsis

Abstract

The preparation of structured antibody layers was shown. For this purpose, a (3‐aminopropyl)‐trimethoxysilane (APTMS) monolayer on a silicon wafer was pholithographically structured. To the APTMS structures, IgG antibodies can easily be attached via glutaraldehyde coupling. To prevent unspecific bonding to the bare silicon substrate, a commercial blocking agent has to be used.