For relative protein quantitation by mass spectrometry we metabolically labeled E. coli bacteria with (15)N-enriched diets. Proteins extracted from (15)N-labeled and unlabeled E. coli bacteria were mixed, separated by two-dimensional gel electrophoresis and enzymatically digested. The resulting tryp
✦ LIBER ✦
Liquid Chromatography−Mass Spectrometry and 15 N Metabolic Labeling for Quantitative Metabolic Profiling
✍ Scribed by Lafaye, Alexandra; Labarre, Jean; Tabet, Jean-Claude; Ezan, Eric; Junot, Christophe
- Book ID
- 120972511
- Publisher
- American Chemical Society
- Year
- 2005
- Tongue
- English
- Weight
- 245 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0003-2700
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