Liquid chromatographic/electrospray ionization tandem mass spectrometric analysis for the quantification of buprenorphine, norbuprenorphine, buprenorphine-3-β-D-glucuronide and norbuprenorphine-3-β-D-glucuronide in human plasma

✍ Scribed by Constance M. Murphy; Marilyn A. Huestis

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 103 KB
• Volume: 40
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.776

✦ Synopsis

Abstract

A liquid chromatographic/electrospray ionization tandem mass spectrometric method for the quantification of the synthetic opiate buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine‐3‐β‐D‐glucuronide (BUP‐3‐G) and norbuprenorphine‐3‐β‐D‐glucuronide (NBUP‐3‐G) in human plasma was developed and validated. Identification and quantification were based on the following transitions: m/z 468 to 396 and 414 for BUP, m/z 414 to 326 and 340 for NBUP, m/z 644 to 468 for BUP‐3‐G and m/z 590 to 414 for NBUP‐3‐G. Calibration by linear regression analysis utilized deuteratated internal standards and a weighting factor of 1/x. The method was accurate and precise across a linear dynamic range of 0.6–50.0 ng ml^−1^. Pretreatment of plasma samples using solid‐phase extraction was sufficient to limit matrix suppression to <30% for all four analytes. The method proved to be suitable for the quantification of BUP and the related metabolites in plasma samples collected from BUP‐maintained study participants. Copyright © 2004 John Wiley & Sons, Ltd.