LC–ESI-MS/MS analysis for the quantification of morphine, codeine, morphine-3-β-D-glucuronide, morphine-6-β-D-glucuronide, and codeine-6-β-D-glucuronide in human urine

✍ Scribed by Constance M. Murphy; Marilyn A. Huestis

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 109 KB
• Volume: 40
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.921

✦ Synopsis

Abstract

A liquid chromatographic‐electrospray ionization‐tandem mass spectrometric method for the quantification of the opiates morphine, codeine, and their metabolites morphine‐3‐β‐D‐glucuronide (M‐3‐G), morphine‐6‐β‐D‐glucuronide (M‐6‐G) and codeine‐6‐β‐D‐glucuronide (C‐6‐G) in human urine has been developed and validated. Identification and quantification were based on the following transitions: 286 to 201 and 229 for morphine, 300 to 215 and 243 for codeine, 644 to 468 for M‐3‐G, 462 to 286 for M‐6‐G, and 476 to 300 for C‐6‐G. Calibration by linear regression analysis utilized deuterated internal standards and a weighting factor of 1/X. The method was accurate and precise across a linear dynamic range of 25.0 to 4000.0 ng/ml. Pretreatment of urine specimens using solid phase extraction was sufficient to limit matrix suppression to less than 40% for all five analytes. The method proved to be suitable for the quantification of morphine, codeine, and their metabolites in urine specimens collected from opioid‐dependent participants enrolled in a methadone maintenance program. Copyright © 2005 John Wiley & Sons, Ltd.