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Liquid chromatographic separation of some PTH-amino acids

✍ Scribed by Ravi Bhushan; Rachna Agarwal

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
209 KB
Volume
12
Category
Article
ISSN
0269-3879

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✦ Synopsis

Liquid chromatographic studies on the separation of ten PTH-amino acids were carried out using normal phase untreated silica gel plates, C-18 RP precoated plates and RP-HPLC. Resolution of a complex mixture of PTH-amino acids was achieved using all the three types. Certain new successful solvent systems have been worked out in each case. HPLC was carried out with Lichrosphere 100 RP-18 (5 mm) column. Acetonitrile and sodium acetate buffer of pH 4.0 was used for reversed phase chromatography while for normal phase TLC combinations of chloroform-acetonitrile and chloroform-tetrahydrofuran were applied.

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