Liquid chromatographic enantiomer resolution of N-fluorenylmethoxycarbonyl α-amino acids and their ester derivatives on polysaccharide-derived chiral stationary phases

✍ Scribed by Yin Hua Li; Wonjae Lee

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 101 KB
• Volume: 28
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200500183

✦ Synopsis

Abstract

The liquid chromatographic enantiomer separation of N‐fluorenylmethoxycarbonyl (FMOC) protected α‐amino acids and their ethyl ester derivatives was performed on polysaccharide‐derived chiral stationary phases, Chiralcel OD, Chiralpak AD, and Chiralpak AS. In general, Chiralcel OD and Chiralpak AD showed good performance for resolution of N‐FMOC α‐amino acids and their ethyl esters, respectively. All investigated N‐FMOC α‐amino acid enantiomers were baseline separated on Chiralcel OD or Chiralpak AD, whereas N‐FMOC α‐amino acid ethyl ester enantiomers were baseline resolved (α = 1.15–3.03) on Chiralpak AD, except for two analytes. The L‐enantiomers of all examined FMOC α‐amino acid ethyl ester derivatives are preferentially retained on Chiralpak AD, while the elution orders of the other enantiomer separations are not consistent.