Liquid Chromatographic Analysis of Vitamin K1in Medical Foods Using Matrix Solid-Phase Dispersion

✍ Scribed by G. William Chase Jr.; R.R. Eitenmiller; A.R. Long

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 117 KB
• Volume: 13
• Category: Article
• ISSN: 0889-1575
• DOI: 10.1006/jfca.2000.0935

✦ Synopsis

A liquid chromatographic method for vitamin K in medical foods is described. The vitamins are extracted from medical food by matrix solid-phase dispersion (MSPD) and quantitated by reversed-phase chromatography with #uorescence detection. Vitamin K is converted to the #uorescent hydroquinone with a post-column zinc reductive reactor. The limit of detection is 6.6 pg and the limit of quantitation is 22 pg on column. Linear response ranged from 45 to 908 pg on column (r"0.998). Recoveries were determined on an analyte-forti"ed zero control reference material (ZRM) for medical foods and averaged 97.9% (n"25) for vitamin K . The method provides a rapid, speci"c and easily controlled assay for the analysis of vitamin K in forti"ed medical foods.