Liposome immunomigration field assay device for Alachlor determination

✍ Scribed by Sui Ti A. Siebert; Stuart G. Reeves; Richard A. Durst

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 903 KB
• Volume: 282
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80214-6

✦ Synopsis

The feasibility of a simple, single-use immunomigration system has been demonstrated, using Alachlor as a model environmental contaminant. In the device, capillary action causes Alachlor and Alachlor-tagged, dye-containing liposomes to migrate through an anti-Ala&or antibody zone, on a plastic-backed nitrocellulose strip, where competitive binding occurs. Unbound liposomes continue migration to a liposome capture zone, where they are quantified either visually or by densitometry. The amount of liposome-entrapped dye that is measured in this zone is directly proportional to the Alachlor concentration in the sample.