Lipids of Daucus carota cell suspension cultures

✍ Scribed by Heinz-Detlef Gregor

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 798 KB
• Volume: 20
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(77)90057-3

✦ Synopsis

In carrot cell suspension cultures ~eater amounts of phospholipid were detected than in carrot toot material, but the major phospholipid classes w~e the same in both materials, and the/r fatty acid Compos/tion wasve~y similax. In centrist to the cultured cells, no significant mounts of free fatty acids and monog!ycegides, as well as diglycefides, could be detected in the carrot root. The fatty ~.cid composition of the majo] lipid classes from cell cultures is reported fog the fWst time in this ~-port. The de~ee of unsaturation was higher in triglycerides and phospholipids than in free fatty acids. In a study of phospholipid biosynthesis, [ 3H ]-glycerol was shown to be incorporated into 4-phospholipids fPE, PC. PG, PS**) to different extents. The highest specific activity was observed in PC and PC;. Five molecular species were isolated from each of the 4 phospho]ipids and analyzed by GC-MS and LSC.