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Lipidomics analysis of essential fatty acids in macrophages

✍ Scribed by Oswald Quehenberger; Aaron Armando; Darren Dumlao; Daren L. Stephens; Edward A. Dennis


Book ID
116838694
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
558 KB
Volume
79
Category
Article
ISSN
0952-3278

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A flow-injection-analysis system has been developed for the determination of essential fatty acids. The enzyme lipoxygenase (EC 1.13.11.12) was immobilized on CNBr-activated Sepharose to form an enzyme column. The oxygen consumption owing to the lipoxygenase-catalyzed conversion from polyunsaturated