✦ LIBER ✦

Enzymatic flow-injection analysis for essential fatty acids

✍ Scribed by M. Schoemaker; F. Spener

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 244 KB
Volume: 19
Category: Article
ISSN: 0925-4005
DOI: 10.1016/0925-4005(93)01100-i

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✦ Synopsis

A flow-injection-analysis system has been developed for the determination of essential fatty acids. The enzyme lipoxygenase (EC 1.13.11.12) was immobilized on CNBr-activated Sepharose to form an enzyme column. The oxygen consumption owing to the lipoxygenase-catalyzed conversion from polyunsaturated fatty acid to hydroperoxy fatty acid was monitored by a flow-through cell containing a Clark-type oxygen electrode. Calibration curves for linoleic and cu-hnolenic acid were nearly identical. A linear correlation was observed between current decrease and fatty acid concentration from 0.1 to 1.2 mM.

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