Lipid composition of heteroploid cells in culture: Effects of prednisolone

✍ Scribed by Robert T. Dell'Orco; George Melnykovych

• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: English
• Weight: 507 KB
• Volume: 76
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040760114

✦ Synopsis

Abstract

Prednisolone‐induced alterations in the content and composition of the total lipids, cholesterol, phospholipids and fatty acids were studied in HeLa S3 cells. After relatively long exposure of the cell cultures to the hormone analog (24 to 72 hours), total cell lipid content was decreased. Partial inhibition of cholesterol and phospholipid metabolism resulted in a shift in the molar ratio of these two lipid constituents. Total fatty acid content was unaffected by prednisolone but there were minor changes in the relative distribution of the individual fatty acids.

The observed decrease in cholesterol turnover after addition of prednisolone was primarily due to the reduced uptake of intact cholesterol from the culture medium. This was compensated, in part, by an increased de novo synthesis of cholesterol from acetate and glucose.