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Fluctuation of alkaline phosphatase activity in synchronized heteroploid cell cultures: Effects of prednisolone

✍ Scribed by George Melnykovych; Carole F. Bishop; Mary Ann B. Swayze

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
445 KB
Volume
70
Category
Article
ISSN
0021-9541

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✦ Synopsis

In two heteroploid cell lines synchronized with thymidine double block, activity of alkaline phosphatase decreased during the 12 hour period preceding mitotic peak. A return to high values was observed during the next 12 hours of synchronous cycle. Prednisolone ( llp, 17a, 21-trihydroxy-1,4-pregnadiene-3,2O-dione), when added to such cell cultures increased alkaline phosphatase activity in one of the cell lines (Henle embryonic intestine) but had the opposite effect on another line (HeLa-S3) in which the enzyme activity was decreased. Neither effect could be demonstrated if the hormone was added at the end of S phase or if cells were arrested in metaphase by vinblastine sulfate.

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