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Lipid composition and effect of amphotericin B on yeast cells ofParacoccidioides brasiliensis

✍ Scribed by Júnia Scares Hamdan; Maria Aparecida Resende

Publisher
Springer Netherlands
Year
1988
Tongue
English
Weight
490 KB
Volume
102
Category
Article
ISSN
0301-486X

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✦ Synopsis

Yeast cells of Paracoccidioides brasiliensis strain SN, were obtained for analysis of lipid composition. Total lipids, phospholipids, sterols, and qualitative sterols and fatty acid composition were determined. Such analysis were made on cells cultured in the presence or absence of amphotericin B and on non proliferating cell suspensions exposed to the antibiotic. Marked alterations in lipid contents were observed in this different conditions. The major alterations were the reduction of total lipids, sterols, and palmitoleic acid in both, proliferating and non proliferating antibiotic exposed cells. The effect of amphotericin B was evaluated also in terms of viability and release of intracellular substances, at different times of exposure. The minimal inhibitory concentration (MIC) determined for that strain of this fungus was 0.2 ~g/mL.

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