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Linker mutagenesis in the gene encoding the periplasmic maltose-binding protein of E. coli

✍ Scribed by DUPLAY, P; BEDOUELLE, H; SZMELCMAN, S; HOFNUNG, M

Book ID
122712416
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
181 KB
Volume
67
Category
Article
ISSN
0300-9084

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## Abstract The __ptsG__ gene that encodes the major glucose transporter of __Escherichia coli,__ II^Glc^, was inserted into a pMALE‐__amp^r^__ expression vector down‐stream of the __malE__ gene which encodes the __E. coli__ maltose‐binding protein (MBP). II^Glc^‐MBP in the 2 h high speed supernata