Linkage and physical mapping of prolactin to porcine chromosome 7

✍ Scribed by A. Vincent; L. Wang; C. K. Tuggle; M. F. Rothschild

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 227 KB
• Volume: 29
• Category: Article
• ISSN: 0268-9146
• DOI: 10.1046/j.1365-2052.1998.00228.x

✦ Synopsis

Comparative mapping studies between human and pig have shown that there is conserved synteny between human chromosome 6 and pig chromosomes 1 and 7, but some gene locations are not well established. Prolactin (PRL), an anterior pituitary hormone, has been mapped to human chromosome 6, and has tentatively mapped to pig chromosome 7 using Southern‐RFLP analysis with a limited number of meioses. To confirm the assignment of prolactin to porcine chromosome 7 by physical and linkage analysis, pig cDNA and human genomic DNA sequences were used to design pig‐specific PCR primers. The primers amplified a fragment of ≈2·8 kb. Two polymorphic restriction sites were identified within this fragment with the restriction endonuclease __Bst__UI. Prolactin was significantly linked to six markers on the published PiGMaP map of pig chromosome 7. Prolactin was physically mapped using a pig × rodent somatic cell hybrid panel. An analysis of these data placed PRL on pig 7p1·1–p1·2 with 100% concordance and was in complete agreement with the linkage data. Both mapping techniques placed PRL in a conserved order with the loci in the syntenic region of human chromosome 6.