Link between drug absorption solubility and permeability measurements in Caco-2 cells
β Scribed by Vaishali Pade; Salomon Stavchansky
- Book ID
- 109389082
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 53 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0022-3549
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Permeability coefficients across monolayers of the human colon carcinoma cell line Caco-2, cultured on permeable supports, are commonly used to predict the absorption of orally administered drugs and other xenobiotics. This protocol describes our method for the cultivation, characterization and dete
Measurement of permeation across a monolayer of the human adenocarcinoma cell line, Caco-2, is a popular surrogate for a compound's permeation across the human intestinal epithelium. Taken alone, however, Caco-2 permeability has certain limitations in the prediction of the extent of absorption of an