Linear dichroism and fluorescence polarization of diphenyl polyenes in stretched polyethylene films

✍ Scribed by L.V. Natarajan; Fredrick M. Stein; Robert E. Blankenship; Raymond Chang

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 363 KB
• Volume: 95
• Category: Article
• ISSN: 0009-2614
• DOI: 10.1016/0009-2614(83)80346-7

✦ Synopsis

A study of the linear dichroism and fluorescence polarization of diphenylpolyenes (CeHs -(CLi=CH)]r-Cells) wirh II = 1.2, X.4.6. 8 in stretched polyethylene films sho\vs tllat the polyencs orient in the anisotropic matris with their Iransition dipole (emission and absorption, n + IT*) aligned with tltc direction of stretch. The masimum dichroic tatio is observed for 1.6diphcnyl-1.3,5-hcsatricnc (DPH). with n = 3, whcrcas 111e values are substantially lower for II < 3 or rr > 3. The hi& orientation of DPH and poor alignment of the hi&r polycnes may be accounted for in ternls of the growing flexibility of the polyene chains with increasin, n chain length. The results justify the use of DPH as a convenient fluorescence probe for biological und synthetic membranes.