The influence of a super-limited tryptic hydrolysis on physicochemical and surface functional properties of faba bean legumin has been studied using size-exclusion HPLC, SDS-PAGE, UV and fluorescence spectroscopy, fluorescence probe techniques, surface tension measurements as well as determination o
Limited tryptic hydrolysis of faba bean legumin: Conformational stability of legumin-T
โ Scribed by Th. Henning; S. Dudek; K.D. Schwenke; A.N. Danilenko; E.E. Braudo
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 50 KB
- Volume
- 42
- Category
- Article
- ISSN
- 1613-4125
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract Oneโdimensional and twoโdimensional SDSโPAGE and Hydrophobic Cluster Analysis (HCA) were used for investigating the course of limited tryptic hydrolysis of faba bean legumin. SDSโPAGE revealed the formation of an โunequalโ subunit pattern, characterized by 30 and 50 kDa subunits, which
Changes of the surfxe tension at a planar interface and of the emulsifying properties of tryptically digested legumin from faba beans (enzyme/substrate ratio = 1/40) were studied in terms of time of hydrolysis (0-48 h). Simultaneously, molecular changes of legumin were studied by determination of li
## Abstract Kinetics and mechanism of proteolysis of broad bean (__Vicia faba__ L.) legumin by trypsin in concentrated solutions at different enzymeโsubstrate ratios (__E/S__ = 1/40, 1/100, 1/200, 1/1000) were studied. By the method of HPLC it was established that during proteolysis process (__E/S_