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Limited proteol ysis of coupling factor-latent ATPase from Mycobacterium phlei. Effects of different enzymes and modifying agents

✍ Scribed by Carolyn J. Ritz-Gold; Arnold F. Brodie


Book ID
115717321
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
596 KB
Volume
547
Category
Article
ISSN
0005-2728

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## Abstract The membrane bound coupling factor‐latent ATPase was solubilized from the membrane vesicles of Mycobacterium phlei by using 0.25 M sucrose or low ionic strength buffer. Purification of the solubilized enzyme by use of Sepharose‐ADP conjugate gel yielded a homogenous preparation of laten