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Effects of trypsin treatment on the structure and function of solubilized coupling factor-latent ATPase from Mycobacterium phlei

✍ Scribed by Carolyn J. Ritz; Arnold F. Brodie


Book ID
118311990
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
408 KB
Volume
75
Category
Article
ISSN
0006-291X

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Purification and properties of membrane-
✍ Kalra, Vijay K. ;Lee, Soon-Ho ;Ritz, Carolyn J. ;Brodie, Arnold F. πŸ“‚ Article πŸ“… 1975 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 579 KB

## Abstract The membrane bound coupling factor‐latent ATPase was solubilized from the membrane vesicles of Mycobacterium phlei by using 0.25 M sucrose or low ionic strength buffer. Purification of the solubilized enzyme by use of Sepharose‐ADP conjugate gel yielded a homogenous preparation of laten