Leishmania: Methods and Protocols

✍ Scribed by Joachim Clos

Publisher: Springer New York; Humana
Year: 2019
Tongue: English
Leaves: 375
Series: Methods in Molecular Biology 1971
Edition: 1st ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide.

✦ Table of Contents

Front Matter ....Pages i-xiii
Host-Free Systems for Differentiation of Axenic Leishmania (Dan Zilberstein, Roni Nitzan Koren)....Pages 1-8
Phylogenetic Studies (Katrin Kuhls, Isabel Mauricio)....Pages 9-68
A Guide to Next Generation Sequence Analysis of Leishmania Genomes (Hideo Imamura, Jean-Claude Dujardin)....Pages 69-94
Quantitative RNA Analysis Using RNA-Seq (Peter J. Myler, Jacqueline A. McDonald, Pedro J. Alcolea, Aakash Sur)....Pages 95-108
Ribosome Profiling in Trypanosomatids (Amelie J. Kraus, Raúl O. Cosentino)....Pages 109-122
Cosmid Library Construction and Functional Cloning (Joachim Clos, Dorothea Zander-Dinse)....Pages 123-140
Cos-Seq: A High-Throughput Gain-of-Function Screen for Drug Resistance Studies in Leishmania (Jade-Eva Potvin, Philippe Leprohon, Elodie Gazanion, Mansi Sharma, Christopher Fernandez-Prada, Marc Ouellette)....Pages 141-167
Gene Replacement by Homologous Recombination (Henner Zirpel, Joachim Clos)....Pages 169-188
LeishGEdit: A Method for Rapid Gene Knockout and Tagging Using CRISPR-Cas9 (Tom Beneke, Eva Gluenz)....Pages 189-210
DiCre-Based Inducible Disruption of Leishmania Genes (Samuel M. Duncan, Elmarie Myburgh, Eliza V. Alves-Ferreira, Jeremy C. Mottram)....Pages 211-224
DiCre-Based Inducible Gene Expression (Jeziel D. Damasceno, Luiz R. O. Tosi, Renato E. R. S. Santos, Jeremy C. Mottram)....Pages 225-235
Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments (Eugenia Bifeld)....Pages 237-247
Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR) (Eugenia Bifeld)....Pages 249-263
In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening (Nuno Santarém, Joana Tavares, Anabela Cordeiro-da-Silva)....Pages 265-277
Quantification of Parasite Loads by Automated Microscopic Image Analysis (Carolina Borsoi Moraes, Laura Maria Alcântara)....Pages 279-288
Quantification of Leishmania Parasites in Murine Models of Visceral Infection (Joana Tavares, Nuno Santarém, Anabela Cordeiro-da-Silva)....Pages 289-301
Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis (María Dolores Jiménez-Antón, Montserrat Grau, Ana Isabel Olías-Molero, José Mª Alunda)....Pages 303-314
Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation Versus Chronicity of Disease (Christian Bogdan, Andrea Debus, Heidi Sebald, Baplu Rai, Johanna Schäfer, Stephanie Obermeyer et al.)....Pages 315-349
Establishment, Maintenance of Phlebotomus spp. in the Laboratory, and Infection with Leishmania spp. (Ifhem Chelbi, Elyes Zhioua)....Pages 351-368
Back Matter ....Pages 369-370

✦ Subjects

Medicine & Public Health; Infectious Diseases

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