Lead-induced inhibition of brain adenyl cyclase
โ Scribed by NATHANSON, JAMES A.; BLOOM, FLOYD E.
- Book ID
- 109695511
- Publisher
- Nature Publishing Group
- Year
- 1975
- Tongue
- English
- Weight
- 236 KB
- Volume
- 255
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/255419a0
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๐ SIMILAR VOLUMES
Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane
cAMP binds to surface receptors of Dictyostelium discoideum cells, transducing the signal to adenylate cyclase, guanylate cyclase and to chemotaxis. The activation of adenylate cyclase is maximal after 1 min and then declines to basal levels due to desensitization, which is composed to two component