Large-scale production of human lymphoblastoid interferon
β Scribed by White, Richard J.; Klein, Frederick
- Book ID
- 123158540
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 562 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0305-7372
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Suwnary A procedure for large scale purification of human lymphoblastoid (Namalva) interferon (IFN) is presented. The procedure includes concentration by ultrafiltration, gel filtration on Sephadex G-75 and affinity chromatography on anti-INF antibody (polyclonal or monoclonal columns. The IFN speci
## Abstract We describe the production of marmoset lymphoblastoid cell interferon (IFN). Optimal yields of IFN were obtained when EBVβtransformed lymphoblastoid cell lines (LCL) at a cell density of 1 Γ 10^6^/ml were incubated with 100 HAU Sendai virus/ml for 24 h. Sendaiβvirusβinduced marmoset lym