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Large-scale production of Entamoeba histolytica trophozoites in polyxenic culture

✍ Scribed by W.M. Spice; J.P. Ackers

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
228 KB
Volume
84
Category
Article
ISSN
0035-9203

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p