Stable expression plasmid for high-level production of GroE molecular chaperones in large-scale cultures

Large-scale production of endotoxin-free

Large-scale production of endotoxin-free plasmids for transient expression in mammalian cell culture

✍ Aleksei Rozkov; Bert Larsson; Stefan Gillström; Robert Björnestedt; Stefan R. Sc 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 219 KB 👁 2 views

## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p