Lactose operon mutants
✍ Scribed by József Szeberényi
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Year
- 2002
- Tongue
- English
- Weight
- 41 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1470-8175
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Terms to be familiar with before you start to solve the test: Lactose operon, operator, electrophoretic mobility shift assay, repressor, catabolite activator protein, RNA polymerase, σ‐factor, gel filtration, exclusion limit, void volume (V~0~), elution volume (V~e~), expression plasmid.
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## Abstract Terms to be familiar with before you start to solve the test: tryptophan, operon, operator, repressor, inducer, corepressor, promoter, RNA polymerase, chromosome‐polysome complex, regulatory gene, cis‐acting element, trans‐acting element, plasmid, transformation
The entire Escherichia coli lactose operon was inserted into an E. coli/Corynebacterium glutamicum shuttle vector and introduced into the gram-positive host organism C. glutamicum R 163. Recombinant C. glutamicum strains carrying the lac genes downstream of an efficient promoter displayed rapid grow