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Studies on the utilization of lactose byCorynebacterium glutamicum, bearing the lactose operon ofEscherichia coli

✍ Scribed by W. Brabetz; W. Liebl; K.-H. Schleifer

Publisher: Springer
Year: 1991
Tongue: English
Weight: 645 KB
Volume: 155
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00245357

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✦ Synopsis

The entire Escherichia coli lactose operon was inserted into an E. coli/Corynebacterium glutamicum shuttle vector and introduced into the gram-positive host organism C. glutamicum R 163. Recombinant C. glutamicum strains carrying the lac genes downstream of an efficient promoter displayed rapid growth with lactose as the sole source of carbon. Two prerequisites were necessary to obtain good growth of C. glutamicum R 163 on lactose: presence of the lacY gene in addition to lacZ and an appropriate promoter for efficient transcription in C. glutamicum. The galactose moiety of lactose was not utilized but accumulated in the culture broth. C. glutamicum strains carrying only the lacZ (beta-galactosidase) gene but not lacY (lactose permease) were not able to grow in lactose minimal medium.

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